Olena Sharikadze

Shupyk National Medical Academy of postgraduate education, Ukraine



Biography

Olena Sharikadze has completed his PhD from of Kharkiv National Medical University and Postdoctoral studies from of Kharkiv National Medical University. She is an Associate Professor at Paediatric Department Shupyk National Medical Academy of Postgraduate Education. She is the regular member of UAP, EEACI. She has published more than 30 papers in reputed journals and has been serving as an Editorial Board Member of repute.

Abstract

Introduction: Among modern strategies for preventing allergies in children, attention is paid to the development of new rational diets, control of the internal environment, control of influence of other factors such  as  ecology, food quality control, the treatment the concomitant diseases.  In addition there is another direction is the ability to control microbial condition. 
 
Aim: Study of the possible influence of the symbiotic on the activity level of cytokines in children and the evaluation of the control of the development of food allergy associated with cow's milk proteins.
 
Materials & Methods: 78 children aged one to four months from healthy mothers and women with allergic pathology were evaluated. The stage of our work was to investigate the clinical and prophylactic efficacy of Lactobacillus rhamnosus GG (LGG) in the experimental groups of children by comparative analysis of a number of immunological parameters before and after receiving the appropriate symbiotic. We analysed the levels of TNF-a, IL-10, IL-5 and IL-13 cytokines in faeces in children of all groups using ELISA. Data were determined before the start of therapy and one month later.
 
Results: As it turned out, after four weeks of taking the symbiotic, in children of the 1st and 2nd experimental groups, 1.87 and 1.89 times, respectively, decreased faecal TNF-α (p<0.05). At the same time, no significant changes in the level of TNF-α among patients in groups 3 and 4 who did not receive a symbiotic were observed (p>0.05)
 
Conclusion: Adding symbiotic to complex therapy leads to a shift in the cytokine balance toward the Th1 response by lowering the IL-13 and TNF-alpha and IL-10 activation.